Drake Bell (25)

Peter Parker / Spider-Man (voice)

Misty Lee (35)

Aunt May Parker (voice)

J.K. Simmons (57)

J. Jonah Jameson (voice)

Tyler James Williams (19)

Prowler (voice)

Stan Lee (89)

Unknown

Quinton Flynn (47)

Unknown

Clark Gregg (49)

Phil Coulson (voice)

Adrian Pasdar (46)

Unknown

John DiMaggio (43)

Unknown

Steven Weber (51)

Unknown

Caitlyn Taylor Love (17)

Ava Ayala / White Tiger (voice)

Tom Kenny (49)

Dr. Otto Octavius / Bentley Wittman (voice)

Fred Tatasciore (43)

Unknown

Rob Paulsen (56)

Unknown

Travis Willingham (30)

Unknown

Troy Baker (36)

Unknown

Dante Basco (36)

Flash Thompson (voice)

Chi McBride (50)

Nick Fury (voice)

Greg Cipes (32)

Danny Rand / Iron Fist (voice)

Logan Miller (20)

Sam Alexander / Nova (voice)

Ogie Banks (38)

Luke Cage / Power Man (voice)

Kevin Michael Richardson (47)

/ Groot (voice)

Clancy Brown (53)

/ Taskmaster (voice)

Tara Strong (39)

/ Mary Jane (voice)

Matt Lanter (29)

Harry Osborn (voice) / Flash Thompson / Agent Venom / Harry Osborn (voice)

Ross Lynch (16)

Jack Russell / Werewolf by Night (voice)

Cam Clarke (54)

Captain Ultra/Piledriver (voice)

Mark Hamill (60)

Arnim Zola (voice) / Nightmare / Shao Lao (voice) / Nightmare / Ox (voice)

James Marsters (49)

Korvac/Chitauri #3 (voice)

Billy West (59)

Rocket Raccoon (voice)