Christopher Daniel Barnes (22)

Peter Parker / Spider-Man (voice)

Sara Ballantine

Mary Jane Watson (voice)

Jennifer Hale (22)

Felicia Hardy (voice)

Linda Gary (50)

Aunt May Parker (voice)

Gary Imhoff (42)

Harry Osborn (voice)

Ed Asner (65)

J. Jonah Jameson (voice)

Rodney Saulsberry (38)

Joe "Robbie" Robertson (voice)

Roscoe Lee Browne (72)

Kingpin (voice)

Joseph Campanella (69)

Dr. Curt Connors (voice) / Dr. Curt Connors / Lizard (voice) / Lizard (voice)

Edward Mulhare (71)

Spencer Smythe (voice)

Alyson Court (21)

Jubilee (voice)

Edward Albert (43)

Matt Murdock / Daredevil (voice)

Rue McClanahan (60)

Mrs. Hardy (voice)

Rob Paulsen (38)

Morrie Bench / Hydro-Man (voice)

John Vernon (62)

Doctor Strange (voice)

Lenore Zann (34)

Rogue (voice)

Nichelle Nichols (61)

Miriam (voice)

Amy Hill (41)

Susan Choi (voice)

George Takei (57)

Wong (voice)

Tim Russ (38)

Hobie Brown / Prowler (voice)

Stan Lee (71)

The Man (voice)

Nita Talbot (64)

Anastasia Hardy (voice)

John Beck (51)

Frank Castle / Punisher (voice)

John Phillip Law (57)

John Hardesky / The Cat (voice)

Cam Clarke (37)

Reed Richards / Mr. Fantastic (voice)

Dimitra Arliss (62)

Anastasia Hardy (voice)

Alison Sealy-Smith (35)

Storm (voice)

Walker Edmiston (68)

Bob Frank / Whizzer (voice)

Roger Kern

Gecko (voice)

Carl Ballantine (77)

Lenny Luntz (voice)