Nick Kroll (39)

Nick Birch / Maury the Hormone Monster / Coach Steve (voice)

John Mulaney (35)

Andrew Glouberman (voice)

Jessi Klein (42)

Jessi Glaser (voice)

Jason Mantzoukas (44)

Jay Bilzerian (voice)

Maya Rudolph (45)

Diane Birch / Constance the Hormone Monstress (voice)

Fred Armisen (50)

Elliot Birch (voice)

Jordan Peele (38)

Ghost of Duke Ellington (voice)

Pamela Adlon (51)

Sonya the Lovebug (voice)

Andrew Rannells (39)

Matthew MacDell (voice) / Matthew (voice) /

Ayo Edebiri (21)

Missy Foreman-Greenwald (voice) / Missy Foreman-Greenwald / Mosaic Missy (voice)

Jenny Slate (35)

Missy Foreman-Greenwald (voice) /

Jessica Chaffin (43)

Shannon Glaser (voice) / / Shannon Glaser / Grace / Intellect Sphinx (voice)

Brandon Kyle Goodman

Walter the Lovebug (voice) / / Walter (voice)

Jon Hamm (46)

Scallops (voice)

Kristen Bell (37)

Pam the Pillow (voice) / Uninterested Girl (voice)

Jon Gabrus (35)

Movie Theater Customer (voice)

Anna Graves (39)

Mrs. Albert (voice)

Nasim Pedrad (35)

Fatima (voice)

Mae Whitman (29)

Tallulah Levine (voice)

Chrissy Guerrero

Choir Girl (voice)

Philip Quinaz

Tanya Alvarez (voice)

Ashley London (22)

The Virgin Mary / Waitress (voice) / (voice) / Kitty / DNA Expert / Pornscape Residents (voice) / Skank (voice)

John Gemberling (36)

Tyler the Hormone Monster (voice) / Security Guard / Bus Driver's Son (voice) / Tyler the Hormone Monster / Dot #1 (voice)

Harvey Fierstein (65)

Older Guy Town Resident (voice)

Jerry Minor (47)

Man #3 / Singing Fish (voice)

Joe Wengert

/ Caleb (voice) / Caleb / Lump Humpman (voice) / Stan the Hormone Monster / Ambition Gremlin (voice) / Mr. Albert (voice) / Caleb / Rewind Shirt Kid / Go-Gurt Burglar (voice) / Steven Tyler Glade Plug-In / Zombie (voice) / Caleb / Go-Gurt Burglar (voice) / Caleb / Dot #3 / Leore Paulblart (voice) / Teacher (voice) / Caleb / Go-Gurt Thief / Teacher (voice) / Caleb / Pornscape Residents (voice) / Caleb / IUD (voice)

Piotr Michael (29)

Siamese Twin #2 (voice)

Ileen Goldsmith

Jessi Glaser - singing double / Judd's Girlfriend (voice) / (voice)

Marisa Lark Wallin

Reese's Wertherspoon / Woman (voice)

Craig Robinson (45)

Pubic Hair #2 (voice) / Harvey Brooks (voice) / Craig (voice) / End Credits Singer (voice)