Patton Oswalt (48)

Uncle Ben (voice)

Jim Cummings (64)

Hammerhead (voice)

Nadji Jeter (20)

Miles Morales (voice) / Security Robot (voice)

Alastair Duncan (59)

Adrian Toomes (voice) / Thug #3 (voice)

Robbie Daymond (35)

Peter Parker / Spider-Man (voice) / Bully #1 / Hot Dog Announcer / Ticket Taker (voice) / Cop #1 (voice)

John DiMaggio (48)

Raymond Warren (voice) / Security Guard/Customer (voice) / Policeman (voice)

Scott Menville (46)

Doc Ock / Otto Octavius (voice) / Grady Scraps (voice) / The Living Brain / Cop #2 (voice)

Nancy Linari (62)

Aunt May (voice) / Landlady (voice) / Woman Bystander (voice) / Bomb Voice (voice)

Max Mittelman (26)

Harry Osborn (voice) / Police Officer #1/Osborn Technician #2/Police Officer #2 (voice) / Male Clerk/Guy in Line (voice) / Thug #2 / Little Kid (voice)

Laura Bailey (36)

Gwen Stacy (voice) / Tully (voice) / Osborn Recruiter #2 (voice)

Josh Keaton (38)

Norman Osborn (voice) / Prison Guard (voice) / Unidentified Voice / Police Officer (voice) / Hologram (voice)

Melanie Minichino (33)

Anya Corazon (voice) / Chaperone (voice) / Female Clerk (voice) / Student #1 (voice) / Helicopter Pilot (voice)

Ben Diskin (34)

Flash Thompson / Police Officer #2/Hoodlum #1/Police Officer #4 (voice) / Student #2 (voice) / Osborn Recruiter #1 (voice) / Flash Thompson (voice)

Fred Tatasciore (49)

Max Modell (voice) / Trucker/Mr. Slott (voice) / Police Officer #1 (voice) / Interviewer (voice) / Thug #1 / Father (voice) / Bystander (voice)

Cameron Boyce (18)

Herman Schultz / Shocker (voice)

Nathaniel J. Potvin (17)

Prowler (voice)

Richard Doyle (72)

Police Officer #3 (voice)

Zeno Robinson (23)

Randy Robertson (voice)

Daniel Hagen

Cop (voice)

Jason Spisak (43)

Alistair Smythe (voice) / Alistair Smythe / Scorpion (voice) / Audience Member (voice) / Scorpion / Purse Snatcher / Driver (voice)

Matthew Mercer (35)

Aleksei Sytsevich/Comic Book Guy (voice) / Aleksei Sytsevich / Rhino / Reporter (voice)

Grey DeLisle (43)

Black Cat/Nurse/Mom (voice) / Black Cat (voice)

Travis Willingham (36)

Sandman / Flint Marko (voice)

Sofia Carson (24)

Sand Girl (voice) / Keemia (voice)

Yuri Lowenthal (46)

Clayton Cole / Clash (voice) / Curt Connors (voice) / Nocturnal (voice)

Jeremy Shada (20)

Ross Caliban (voice)

Bob Joles (58)

J. Jonah Jameson (voice)

Aaron Abrams (39)

Tinkerer (voice) / Tinkerer / Police Officer (voice) / Tinkerer / Old Man (voice)

Sumalee Montano (45)

Yuri Watanabe (voice)

Felicia Day (38)

Mary Jane (voice)