Peter Paige (38)

Steve Ball (voice)

Taylor M. Dooley

Dana Bernstein (voice)

Wilson Cruz (33)

Evan Martinez (voice) / Charo (voice)

Alan Cumming (42)

Chuck Masters (voice) / Chuck (voice) / Rupert (voice) / Zeke (voice)

Will Matthews

Rick Brocka Jr. (voice) / F. Cornelious Hampton-Hampton III (voice) / Echinacea (voice) / Timmy (voice) / Echinacea / Timmy (voice)

Q. Allan Brocka (34)

Frank Nerdlinger / Michaela (voice) / Monsterbator Promoter / Announcer / Receptionist / Nerds (voice) / Falaysha (voice) / Falaysha / Ricky Brocka Sr. (voice) / Announcer / Protester / Straight Guy / Club Girl (voice) / Flaxseed / Greenpeacer / "We're All Going to Die" Guy (voice) / Ricky Brocka Sr. (voice) / Condom Student (voice) / Father Figure / Muddy Frat Boy / Javelin Thrower (voice) / Wiener / Ricky Brocka Sr. / Stud #1 / Stud #2 (voice) / Ricky Brocka Sr. / Republican / Hysterical Klansman (voice)

Billy West (55)

Dr. Hunk (voice) / Log Cabin Tom (voice) / Carlton (voice) / Anderson Pooper (voice) / Ryan Peefest (voice) / Hunter (voice) / Log Cabin Tom / Dylan Rambrick (voice) / George W. Bush (voice) / Asspounder / Carlton (voice) / Crazy Raul / Straight Guy (voice) / Additional Voices (voice)

Kimberley Locke (29)

Steamroom Sally (voice)

RuPaul (46)

Tyler (voice)

Alec Mapa (42)

Uncle Bakla (voice)

Robert Lopez

Ben Affleck (voice)

Lorna Luft (54)

Joanna (voice)

Laraine Newman (55)

Chastity Bono / Announcer / Additional Voices (voice)

Margaret Cho (38)

Condie Ling (voice) / Condie Ling / Jessica (voice) / Condie Ling / Kathy Griffin (voice)

Mitch Morris (27)

Preston Fessler (voice)

Mark Hamill (55)

Saul (voice)

Perez Hilton (29)

Self (voice)

Jessica-Snow Wilson

Kirsten (voice) / Kirsten / Mary Jane (voice)

Jim J. Bullock (52)

Jacques-Jean / Emerald Joe (voice)

Lori Alan (40)

Ivory / Dr. Joy / Additional Voices (voice) / Dixie / Ivory (voice) / Dixie / Nerd (voice) / Dixie (voice) / Dixie / Mail Woman / Eunice (voice) / Ivory / Dixie (voice) / Ivory / Nerds (voice)

Emily Brooke Hands

Kirsten (voice) / Mrs. Peghole / Rita (voice) / Straight Woman / Protestor (voice) / Heather (voice)

Jennifer Coolidge (45)

Fannie Mae (voice)

Lance Bass (28)

Self (voice)

Jill Bennett (31)

General B. Traytus (voice)

Andy Dick (41)

Protestor (voice)

Miss Coco Peru (41)

Mother Morally Superior (voice)

Jai Rodriguez (28)

Harry Tubman (voice)

George Takei (70)

Mayor Screwsum / Police Chief (voice)

Bruce Vilanch (58)

Bruce Bandersnatch (voice)

Liza del Mundo (32)

Ebony / Arugula (voice) / Minda / Pussy / Ebony (voice) / Clever Housewife (voice) / Minda / Clever Dead Housewife (voice) / Pussy / Ursula (voice) / Pussy (voice) / Migrant Worker (voice) / Pussy / Ebony / Nerds (voice) / Minda / Pussy / Additional Voices (voice) / Pussy / Myrna (voice) / Arugula (voice) / Minda / Pussy / Clever Dead Housewife (voice)