Angelle Brooks (35)

Indira 'Indy' Daimonji (voice)

Neil Patrick Harris (30)

Peter Parker / Spider-Man (voice)

Lisa Loeb (35)

Mary Jane Watson (voice)

Ian Ziering (39)

Harry Osborn (voice)

Ed Asner (73)

Officer Barr (voice)

Virginia Madsen (41)

Silver Sable (voice)

Ethan Embry (25)

Max Dillon / Electro (voice) / Max Dillon (voice)

Michael Dorn (50)

Kraven the Hunter (voice)

James Marsters (40)

Sergei (voice)

Clancy Brown (44)

Raymond (voice) / Policeman (voice)

Michael Clarke Duncan (45)

Wilson Fisk / The Kingpin (voice)

Stan Lee (80)

Frank Elson (voice)

Rob Zombie (38)

Dr. Curt Connors / The Lizard (voice)

Gina Gershon (41)

Shikata (voice)

John C. McGinley (43)

Richard Damien (voice)

Jane Lynch (42)

Oscorp Executive (voice)

Tara Strong (30)

Christina (voice)

Devon Sawa (24)

Flash Thompson (voice)

Jeffrey Combs (48)

Professor (voice) / Dr. Zellner (voice)

Karen Maruyama (45)

Shadowy Woman / Flower Vendor (voice) / Head Protestor (voice)

Jeremy Piven (37)

Roland Gaines (voice)

Keith David (47)

Agent Mosely (voice)

Kathy Griffin (42)

Roxanne Gaines (voice)

Jennifer Hale (31)

Female News Announcer (voice) / Female Reporter (voice)

Julie Nathanson (30)

Sally Johnson (voice)

Rino Romano (34)

Muang / Drip Den guy (voice) / Doug's friend (voice)

Keith Carradine (53)

J. Jonah Jameson (voice)

Eve (24)

Cheyenne Tate / The Talon (voice)

Brendan Connolly (45)

Aleksei (voice)

James Horan (48)

Henchman (voice)