Hudson Thames (30)

Peter Parker / Spider-Man (voice)

Colman Domingo (55)

Norman Osborn (voice)

Eugene Byrd (49)

Lonnie Lincoln (voice)

Grace Song (23)

Nico Minoru (voice)

Zeno Robinson (31)

Harry Osborn (voice) / Mr. Campbell (voice)

Kari Wahlgren (47)

May Parker (voice) / Roxanna Volkov (voice) / Mrs. Eberhardt (voice)

Charlie Cox (42)

Daredevil (voice)

Robin Atkin Downes (48)

Doctor Strange (voice)

Cathy Ang (29)

Pearl Pangan (voice)

Ogie Banks (51)

Pizza Shop Owner (voice)

Andre Robinson (20)

Additional Voices (voice)

Arthur Ortiz (42)

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Diamond White (25)

Additional Voices (voice)

Griffin Puatu (27)

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Isabella Acres (23)

Additional Voices (voice)

Nick Carson

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Noah Kaye Bentley

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Oscar Camacho

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ViviAnn Yee

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Terri Douglas (51)

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Aleks Le (25)

Amadeus Cho (voice)

Paul F. Tompkins (56)

Bentley Wittman (voice)

Jake Green

Butane (voice)

Erica Luttrell (42)

Asha (voice) / Emma (voice) / Mrs. Lincoln (voice) / Emma / Mrs. Lincoln (voice)

Phil LaMarr (58)

Mr. Taylor (voice) / Mr. Taylor / Mr. Lincoln (voice) / Mr. Lincoln (voice)

Anairis Quinones (27)

Carmilla Black (voice) / Maria Vasquez (voice)

Kellen Goff (29)

Symbiotic Alien (voice) / Vincent Patillo (voice) / Vincent Patilio (voice)

Ettore Ewen (38)

Bulldozer (voice)

Roger Craig Smith (49)

Coach Grayfield (voice) / Dmitri Smerdyakov / Chameleon (voice) / Dimitri Smerdyakov (voice) / James 'Speed Demon' Sanders (voice) / Dmitri Smerdyakov (voice)

Alex Désert (56)

Grift (voice)