Benjamin Valic

Peter Parker / Spider-Man (Spidey) (voice)

Alkaio Thiele (11)

Peter Parker / Spider-Man (Spidey) (voice)

Lily Sanfelippo

Gwen Stacy / Ghost-Spider (voice)

Audrey Bennett

Gwen Stacy / Ghost-Spider (voice)

Jakari Fraser

Miles Morales / Spider-Man (Spin) (voice)

Carter Young (9)

Miles Morales / Spider-Man (Spin) (voice)

Nick Fisher (8)

Peter Parker / Spider-Man (Spidey) (voice)

Gabrielle Ruiz (36)

Rio Morales (voice)

Kari Wahlgren (44)

Helen Stacy (voice)

Dee Bradley Baker (58)

Trace-E (voice) / Trace-E / CAL (voice)

John Stamos (57)

Tony Stark / Iron Man (voice)

Jaiden Klein

Felicia Hardy / Black Cat (voice)

Stephanie Lemelin (42)

Francine Frye / Electro (voice)

Thomas F. Wilson (62)

William Baker / Sandman (voice)

Melanie Minichino (37)

Aunt May (voice)

Tru Valentino

T'Challa / Black Panther (voice)

Sandra Saad

Kamala Khan / Ms. Marvel (voice)

Armen Taylor

Bruce Banner / Hulk (voice)

Eugene Byrd (45)

Jefferson Morales (voice)

Justin Shenkarow (40)

Aleksei Sytsevich / Rhino (voice)

Bindi Irwin (23)

Isla Coralton (voice)

Sean Giambrone (22)

Hank Pym / Ant-Man (voice)

Maya Tuttle

Janet Van Dyne / Wasp (voice)

Hoku Ramirez

Humberto Lopez / Reptil (voice)

JP Karliak (40)

Norman Osborn / Green Goblin (voice) / Green Goblin (voice)

Sophia Ramos

Gloria Morales (voice)

Kelly Ohanian

Carolyn Trainer / Doctor Octopus (voice) / Carolyn Trainer / Dr. Octopus (voice) / Cyrilla Calypso / Doc Ock / Visitor 2 (voice)

Scott Porter (42)

George Stacy (voice)

Melique Berger

Mayor (voice)

Patrick Stump (37)

Concert Announcer (voice)