David Kaufman (41)

Stuart Little (voice)

Myles Jeffrey (12)

George Little (voice)

Jennifer Hale (31)

Eleanor Little / Martha Little (voice)

Hugh Laurie (43)

Mr. Frederick Little (voice)

Julie Nathanson (29)

(voice)

André Sogliuzzo (36)

Monty (voice)

Kathy Najimy (46)

Margalo (voice)

Kevin Schon (45)

Snowbell (voice)

Rachael Harris (35)

(voice)

Robert Costanzo (60)

(voice)

Quinton Flynn (38)

(voice) / Snowbell (voice)

Dee Bradley Baker (40)

(voice) / Additional Voices (voice)

Debi Derryberry (42)

(voice)

Marc John Jefferies (12)

Will (voice)

Jim Wise (38)

(voice)

Ken Hudson Campbell (40)

(voice)

Daniel Hansen

(voice)

Lacey Chabert (20)

(voice)

Matt Kaminsky (31)

(voice)

Sean Marquette (14)

Zack (voice)

Lauren Tom (41)

(voice)

Edie McClurg (51)

(voice)

Michael Chiklis (39)

Tiger (voice)

Mark Hamill (51)

(voice) / Falcon (voice)

Simbi Khali (31)

(voice)

Carlos Alazraqui (40)

Monty (voice)

Jeff Bennett (40)

Crows (voice)

Pat Fraley (54)

(voice)

Jeffrey Jones (56)

Uncle Crenshaw Little (voice)

Susan Silo (60)

(voice)