Magda Vášáryová (39)

Mary Stuart

Joan Sutherland (61)

Mary Stuart (singing voice)

Kamila Magálová (37)

Queen Elizabeth I

Huguette Tourangeau (47)

Queen Elizabeth I (singing voice)

Milan Kňažko (42)

Earl of Leicester

Luciano Pavarotti (52)

Earl of Leicester (singing voice)

Přemysl Kočí (70)

George Talbot

Roger Soyer (48)

George Talbot (singing voice)

Jan Přeučil (50)

William Cecil

James Morris (40)

William Cecil (singing voice)

Jiřina Jirásková (56)

Hanna Kennedy

Margreta Elkins (57)

Hanna Kennedy (singing voice)

Luděk Munzar (54)

Amias Paulet

Vladimír Dlouhý (29)

Mortimer

Karel Roden (25)

William Davison

Jiří Vala (61)

Melville

Zdeněk Ornest (58)

Kent (uncredited)

Kornelia Boje (45)

Mary Stuart (voice)

Christa Berndl (55)

Queen Elizabeth I (voice)

Randolf Kronberg (45)

Earl of Leicester (voice)

Bettina Schön (61)

Hanna Kennedy (voice)

Hartmut Reck (55)

Amias Paulet (voice)

Martin Umbach (31)

Mortimer (voice)

Michael Cramer (57)

Melville (voice)